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smfish staining bioptechs fcs2 fcs2 stage adapter bioptechs  (Bioptechs inc)

 
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    Bioptechs inc smfish staining bioptechs fcs2 fcs2 stage adapter bioptechs
    Smfish Staining Bioptechs Fcs2 Fcs2 Stage Adapter Bioptechs, supplied by Bioptechs inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bioptechs+fcs2/FCS2+Cooling+Adapter/pm41990751-296-170-172
    Average 96 stars, based on 1 article reviews
    smfish staining bioptechs fcs2 fcs2 stage adapter bioptechs - by Bioz Stars, 2026-09
    96/100 stars

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    other:

    Article Title: HIF1α stabilization in hypoxia is not oxidant-initiated
    Article Snippet: After the islets were loaded into the perifusion chambers, the chamber was sealed and mounted onto the stage of a Nikon Eclipse TE- 200 inverted microscope.

    Article Title: Barrier Immune Memory is Promoted by Intestinal Epithelial Cell Presentation of Injected Bacterial Antigens
    Article Snippet: Samples were mounted in a Bioptechs FCS2 closed-flow chamber (1 mm gasket, Bioptechs 1907-1422-1000) connected to a custom automated fluidics system composed of a peristaltic pump and Hamilton MVP valves.

    Centrifugation:

    Article Title: Acetylation stabilises calmodulin-regulated calcium signalling.
    Article Snippet: .. Cells were grown exponentially at 25 °C for 48 h before being mounted (without centrifugation) onto lectin (Sigma, St. Louis, MI, USA L2380; 1 mg mL−1)-coated coverslips with an a Bioptechs FCS2 (Bioptechs, Butler, PA, USA), fitted onto an ASI motorised stage (ASI, Eugene, OR, USA) on the above system, with the sample holder, objective lens and environmental chamber held at the required temperature. .. Each 3D-maximum projection of volume data was calculated from 21 z-plane images, each 0.2 μm apart, and analysed using METAMORPH and AUTOQUANT X software (Mediacy Cybernetics, Rockville, MD, USA).

    Article Title: Acetylation stabilises calmodulin‐regulated calcium signalling
    Article Snippet: .. Cells were grown exponentially at 25 °C for 48 h before being mounted (without centrifugation) onto lectin (Sigma, St. Louis, MI, USA L2380; 1 mg·mL −1 )‐coated coverslips with an a Bioptechs FCS2 (Bioptechs, Butler, PA, USA), fitted onto an ASI motorised stage (ASI, Eugene, OR, USA) on the above system, with the sample holder, objective lens and environmental chamber held at the required temperature. .. Each 3D‐maximum projection of volume data was calculated from 21 z ‐plane images, each 0.2 μm apart, and analysed using metamorph and autoquant x software (Mediacy Cybernetics, Rockville, MD, USA).

    Imaging:

    Article Title: Oxidants are dispensable for HIF1α stability in hypoxia
    Article Snippet: .. Real-time imaging experiments were carried out while islets were perifused using a commercially available temperature-controlled Bioptechs FCS2, which is a closed system, parallel plate flow chamber (Butler, PA) as previously described ( ). .. After the islets were loaded into the perfusion chambers, the chamber was sealed and mounted onto the stage of a Nikon Eclipse TE-200 inverted microscope.

    Article Title: HIF1α stabilization in hypoxia is not oxidant-initiated
    Article Snippet: .. Real-time imaging experiments were carried out while islets were perifused using a commercially available temperature-controlled Bioptechs FCS2, a closed system, parallel plate flow chamber (Butler, PA) as previously described ( ). .. After the islets were loaded into the perifusion chambers, the chamber was sealed and mounted onto the stage of a Nikon Eclipse TE-200 inverted microscope.

    Article Title: Roles of P-body factors in Candida albicans filamentation and stress response.
    Article Snippet: .. For PB imaging in live cells, strains were grown overnight in a rotator at 30°C in CM, diluted to an OD600 of 0.1, and subcultured (30°C, CM, 300 RPM) for 4 hours before loading into an Bioptechs FCS2 with a coverslip coated with 2% ConA to maintain desired temperature. ..

    Article Title: Roles of P-body factors in Candida albicans filamentation and stress response
    Article Snippet: .. For PB imaging in live cells, strains were grown overnight in a rotator at 30°C in CM, diluted to an OD 600 of 0.1, and subcultured (30°C, CM, 300 RPM) for 4 hours before loading into an Bioptechs FCS2 with a coverslip coated with 2% ConA to maintain desired temperature. ..



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    smfish staining bioptechs fcs2 fcs2 stage adapter bioptechs  (Bioptechs inc)
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    A) Schematic of generating rifampin-induced persisters and evaluating persister survival rates. B) Survival rate (mean value ± standard deviation) of E. coli MG1655 cells against ampicillin with no pretreatment ( A ) and rifampin-pretreatment ( R-A ). Data represent results from six independent experiments. C) Motile percentage (mean value ± standard deviation) of mid-exponential phase cells ( M ), rifampin-treated cells ( R ), rifampin-induced persisters ( R-A ). Data represent results from five experimental videos. The total numbers of cells in the five videos are 598 ( M ), 663 ( R ), and 291 ( R-A ), respectively. D - F) Representative trajectories of mid-exponential phase cells ( D ), rifampin-treated cells ( E ), and persisters ( F ) swimming in their original living environment (LB or LB with corresponding antibiotics), imaged in the <t>FCS2</t> chamber. The colored lines show the trajectories of different cells. G , H) Comparison of track mean speed ( G ) and track mean directional change rate ( H ) distributions for mid-exponential phase cells ( M ), rifampin-treated cells ( R ), and persisters ( R-A ). Data were obtained from at least three experimental videos. Sample sizes for mid-exponential phase cell, rifampin-treated cell, and persister are 6358, 6580, and 4610 tracks, respectively.
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    bioptechs fcs2 flow chamber  (Bioptechs inc)
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    A) Schematic of generating rifampin-induced persisters and evaluating persister survival rates. B) Survival rate (mean value ± standard deviation) of E. coli MG1655 cells against ampicillin with no pretreatment ( A ) and rifampin-pretreatment ( R-A ). Data represent results from six independent experiments. C) Motile percentage (mean value ± standard deviation) of mid-exponential phase cells ( M ), rifampin-treated cells ( R ), rifampin-induced persisters ( R-A ). Data represent results from five experimental videos. The total numbers of cells in the five videos are 598 ( M ), 663 ( R ), and 291 ( R-A ), respectively. D - F) Representative trajectories of mid-exponential phase cells ( D ), rifampin-treated cells ( E ), and persisters ( F ) swimming in their original living environment (LB or LB with corresponding antibiotics), imaged in the <t>FCS2</t> chamber. The colored lines show the trajectories of different cells. G , H) Comparison of track mean speed ( G ) and track mean directional change rate ( H ) distributions for mid-exponential phase cells ( M ), rifampin-treated cells ( R ), and persisters ( R-A ). Data were obtained from at least three experimental videos. Sample sizes for mid-exponential phase cell, rifampin-treated cell, and persister are 6358, 6580, and 4610 tracks, respectively.
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    Image Search Results


    A) Schematic of generating rifampin-induced persisters and evaluating persister survival rates. B) Survival rate (mean value ± standard deviation) of E. coli MG1655 cells against ampicillin with no pretreatment ( A ) and rifampin-pretreatment ( R-A ). Data represent results from six independent experiments. C) Motile percentage (mean value ± standard deviation) of mid-exponential phase cells ( M ), rifampin-treated cells ( R ), rifampin-induced persisters ( R-A ). Data represent results from five experimental videos. The total numbers of cells in the five videos are 598 ( M ), 663 ( R ), and 291 ( R-A ), respectively. D - F) Representative trajectories of mid-exponential phase cells ( D ), rifampin-treated cells ( E ), and persisters ( F ) swimming in their original living environment (LB or LB with corresponding antibiotics), imaged in the FCS2 chamber. The colored lines show the trajectories of different cells. G , H) Comparison of track mean speed ( G ) and track mean directional change rate ( H ) distributions for mid-exponential phase cells ( M ), rifampin-treated cells ( R ), and persisters ( R-A ). Data were obtained from at least three experimental videos. Sample sizes for mid-exponential phase cell, rifampin-treated cell, and persister are 6358, 6580, and 4610 tracks, respectively.

    Journal: bioRxiv

    Article Title: Motility Functions as an Essential Defense in the Bacterial Persister Lifecycle

    doi: 10.64898/2025.12.17.694804

    Figure Lengend Snippet: A) Schematic of generating rifampin-induced persisters and evaluating persister survival rates. B) Survival rate (mean value ± standard deviation) of E. coli MG1655 cells against ampicillin with no pretreatment ( A ) and rifampin-pretreatment ( R-A ). Data represent results from six independent experiments. C) Motile percentage (mean value ± standard deviation) of mid-exponential phase cells ( M ), rifampin-treated cells ( R ), rifampin-induced persisters ( R-A ). Data represent results from five experimental videos. The total numbers of cells in the five videos are 598 ( M ), 663 ( R ), and 291 ( R-A ), respectively. D - F) Representative trajectories of mid-exponential phase cells ( D ), rifampin-treated cells ( E ), and persisters ( F ) swimming in their original living environment (LB or LB with corresponding antibiotics), imaged in the FCS2 chamber. The colored lines show the trajectories of different cells. G , H) Comparison of track mean speed ( G ) and track mean directional change rate ( H ) distributions for mid-exponential phase cells ( M ), rifampin-treated cells ( R ), and persisters ( R-A ). Data were obtained from at least three experimental videos. Sample sizes for mid-exponential phase cell, rifampin-treated cell, and persister are 6358, 6580, and 4610 tracks, respectively.

    Article Snippet: To capture the natural swimming behavior of cells in their original living environment, we directly placed 8 μL of bacterial culture onto the Bioptechs FCS2 chamber (0.2 mm plastic pad between slide and coverslip) and used a Nikon ECLIPSE Ti inverted microscope to record bright-field videos at approximately 22 frames per second (FPS).

    Techniques: Standard Deviation, Comparison

    A) Representative trajectories of mid-exponential phase cells swimming in LB after CCCP exposure, observed in the FCS2 chamber. The colored lines show the trajectories of different cells. B) Survival rate (mean value ± standard deviation) of E. coli cells against ampicillin with exposure to CCCP before rifampin ( C-R-A ), exposure to CCCP and rifampin together ( CR-A ), exposure to CCCP and ampicillin together after rifampin ( R-CA ), and 15% w/v Ficoll in culture medium upon rifampin exposure ( 15%FR-A ). Data represent results from six independent experiments. C) Membrane potential (mean value ± standard deviation) of mid-exponential phase cells treated with CCCP ( CCCP ), experiencing no treatment ( M ), treated with 5% ( 5%F ), 10% ( 10%F ), and 15% ( 15%F ) w/v Ficoll, as indicated by the red/green median fluorescence intensity ratio measured by flow cytometry. Data represent results from at least three independent experiments. D) Representative trajectories of mid-exponential phase cells swimming in LB with 15% w/v Ficoll, imaged in the FCS2 chamber. The colored lines show the trajectories of different cells. E) Two-dimensional distribution correlating track mean speed and track mean directional change rate for mid-exponential phase cells in LB with 15% w/v Ficoll, with corresponding marginal distributions. The sample size is 5374 tracks, obtained from seven videos. F) Relationship between rifampin-induced survival rate against ampicillin (mean) and swimming speed (mean) for cells under different conditions: M , 15%F , and CCCP .

    Journal: bioRxiv

    Article Title: Motility Functions as an Essential Defense in the Bacterial Persister Lifecycle

    doi: 10.64898/2025.12.17.694804

    Figure Lengend Snippet: A) Representative trajectories of mid-exponential phase cells swimming in LB after CCCP exposure, observed in the FCS2 chamber. The colored lines show the trajectories of different cells. B) Survival rate (mean value ± standard deviation) of E. coli cells against ampicillin with exposure to CCCP before rifampin ( C-R-A ), exposure to CCCP and rifampin together ( CR-A ), exposure to CCCP and ampicillin together after rifampin ( R-CA ), and 15% w/v Ficoll in culture medium upon rifampin exposure ( 15%FR-A ). Data represent results from six independent experiments. C) Membrane potential (mean value ± standard deviation) of mid-exponential phase cells treated with CCCP ( CCCP ), experiencing no treatment ( M ), treated with 5% ( 5%F ), 10% ( 10%F ), and 15% ( 15%F ) w/v Ficoll, as indicated by the red/green median fluorescence intensity ratio measured by flow cytometry. Data represent results from at least three independent experiments. D) Representative trajectories of mid-exponential phase cells swimming in LB with 15% w/v Ficoll, imaged in the FCS2 chamber. The colored lines show the trajectories of different cells. E) Two-dimensional distribution correlating track mean speed and track mean directional change rate for mid-exponential phase cells in LB with 15% w/v Ficoll, with corresponding marginal distributions. The sample size is 5374 tracks, obtained from seven videos. F) Relationship between rifampin-induced survival rate against ampicillin (mean) and swimming speed (mean) for cells under different conditions: M , 15%F , and CCCP .

    Article Snippet: To capture the natural swimming behavior of cells in their original living environment, we directly placed 8 μL of bacterial culture onto the Bioptechs FCS2 chamber (0.2 mm plastic pad between slide and coverslip) and used a Nikon ECLIPSE Ti inverted microscope to record bright-field videos at approximately 22 frames per second (FPS).

    Techniques: Standard Deviation, Membrane, Fluorescence, Flow Cytometry